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SCIENCE

The Claim

A quantitative PCR (qPCR) assay has been developed and validated to detect Gastrodiscoides hominis DNA in clinical or environmental samples.

Verified by Lenz ·

The Short Version

The evidence does not support the existence of a developed and validated qPCR assay for Gastrodiscoides hominis. Sources that directly discuss this parasite describe microscopy-based diagnosis and, at most, conventional PCR for sequence characterization. The qPCR papers in the record concern other organisms and cannot substantiate a G. hominis-specific assay in clinical or environmental samples.

Caveats

  • Do not treat conventional PCR or ITS sequencing as evidence of a validated qPCR diagnostic assay; they are different methods with different evidentiary requirements.
  • Assays developed for other parasites or pathogens are not proof that an equivalent assay exists for Gastrodiscoides hominis.
  • The claim overstates scope by asserting validated detection in clinical or environmental samples without any direct validation data for either sample type.

The Receipts

  1. Gastrodiscoides hominis - medtigo

    medtigo

  2. Molecular characterization of Gastrodiscoides hominis (Trematoda: Digenea) inferred from ITS rDNA sequence analysis

    PubMed

  3. Development of a TaqMan qPCR assay for the detection and quantification of Gnomoniopsis castaneae, causal agent of brown rot of chestnut kernels

    The American Chestnut Foundation (tacf.org)

  4. Development of a TaqMan qPCR Method for Detecting Angiostrongylus cantonensis in Host Snails

    PMC

  5. Development of a TaqMan qPCR assay for trypanosomatid multi‑host and multi‑environment screening

    PMC

  6. Development of a TaqMan qPCR for the Simultaneous Detection of Turnip Mosaic Virus and Broad Bean Wilt Virus 2

    Microorganisms (MDPI)

  7. Establishment of a TaqMan probe‑based qPCR assay for detecting Enterocytozoon epinepheli in groupers

    PubMed

  8. Gastrodiscoides

    Wikipedia

  9. DPDx - Case #472 - July 2018 - CDC

    CDC

  10. Development of a High-Throughput qPCR Assay for Detecting Waterborne Protozoa and Helminths

    China CDC Weekly

+ 12 more sources — see the full list on Lenz

Filed Under

Gastrodiscoides HominisQuantitative Polymerase Chain Reaction