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IPTG induces expression of T7 RNA polymerase in lac-controlled systems.

The Claim

IPTG induces T7 RNA polymerase to transcribe target sequences in both directions, producing complementary RNA strands that hybridize into double-stranded RNA.

The Short Version

The claim conflates IPTG-induced polymerase expression with a separate RNA byproduct mechanism. IPTG induces expression of T7 RNA polymerase in lac-controlled systems; the polymerase then transcribes targets from a T7 promoter. Purified T7 RNA polymerase can also generate complementary antisense RNA that forms double-stranded RNA during in vitro transcription, but this is conditional, aberrant activity. The evidence does not establish that IPTG induces bidirectional target transcription or the resulting double-stranded RNA production.

Caveats

  • The double-stranded RNA findings principally concern purified-enzyme reactions in vitro and do not establish the claimed mechanism in cells.
  • Sources 3, 5 and 6 are versions of the same study, not independent confirmations.
  • IPTG used to produce polymerase for an experiment is not evidence that IPTG directs the polymerase's transcriptional activity.

The Receipts

  1. A novel transcription property of SP6 and T7 RNA polymerases: dependence on template structure

    doi.org

  2. Uridine analogs prevent stress granule formation, not by blocking PKR recognition, but by inhibiting the synthesis of T7 RNA polymerase byproducts - PMC

    pmc.ncbi.nlm.nih.gov

  3. An origin of the immunogenicity of in vitro transcribed RNA

    doi.org

  4. Template nicking suppresses promoter-independent antisense transcription in IVT via R-loop-mediated strand displacement

    doi.org

  5. origin of the immunogenicity of in vitro transcribed RNA | Nucleic Acids Research | Oxford Academic

    academic.oup.com

  6. An origin of the immunogenicity of in vitro transcribed RNA - PMC

    pmc.ncbi.nlm.nih.gov

  7. A novel regulation mechanism of the T7 RNA polymerase ...

    nature.com

  8. An engineered T7 RNA polymerase for efficient co-transcriptional capping with reduced dsRNA byproducts in mRNA synthesis - Faraday Discussions (RSC Publishing) DOI:10.1039/D4FD00023D

    pubs.rsc.org

  9. T7 RNA polymerase mediates fast promoter-independent extension of unstable nucleic acid complexes

    pubmed.ncbi.nlm.nih.gov

  10. DNA-terminus-dependent transcription by T7 RNA polymerase and its C-helix mutants - PMC

    pmc.ncbi.nlm.nih.gov

+ 4 more sources — see the full list on Lenz

Filed Under

IPTGT7 RNA Polymerase