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Bacterially produced double-stranded RNA triggers target-specific gene knockdown in Caenorhabditis elegans.

The Claim

Bacterially produced double-stranded RNA is consumed by Caenorhabditis elegans and enters the endogenous RNA interference pathway to reduce expression of the corresponding target gene.

The Short Version

Bacterially produced double-stranded RNA is ingested by Caenorhabditis elegans and triggers target-specific gene knockdown through the worm’s own RNAi machinery. Primary feeding experiments and uptake studies support this mechanism. The wording “endogenous RNA interference pathway” is technically loose: ingested dsRNA triggers exogenous RNAi, which shares components with distinct endogenous RNAi pathways.

Caveats

  • “Endogenous RNAi” has a specific technical meaning; shared machinery does not make endogenous and exogenous pathways identical.
  • Feeding-induced knockdown can be partial and varies by tissue; reduced expression does not imply complete silencing.
  • Several listed sources are duplicate or mirrored versions of the same studies, rather than independent confirmations.

The Receipts

  1. RNA Interference: Systemic RNAi SIDes with Endosomes: Current Biology

    cell.com

  2. Caenorhabditis elegans SID-2 is required for environmental RNA interference

    pnas.org

  3. Specific interference by ingested dsRNA | Nature

    nature.com

  4. Environmental RNA interference in animals

    doi.org

  5. Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes

    doi.org

  6. Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans

    pmc.ncbi.nlm.nih.gov

  7. Reverse genetics *

    ncbi.nlm.nih.gov

  8. Specific RNA Interference in Caenorhabditis elegans by ...

    pmc.ncbi.nlm.nih.gov

  9. Specific RNA Interference in Caenorhabditis elegans by Ingested dsRNA Expressed in Bacillus subtilis | PLOS One

    journals.plos.org

  10. Enhanced neuronal RNAi in C. elegans using SID-1 | Nature Methods

    nature.com

+ 24 more sources — see the full list on Lenz

Filed Under

Caenorhabditis ElegansDouble-Stranded RNARNA Interference

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